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Image Search Results
Journal: Nature communications
Article Title: Comprehensive Interrogation of Natural TALE DNA Binding Modules and Transcriptional Repressor Domains
doi: 10.1038/ncomms1962
Figure Lengend Snippet: a, Design of SOX2 TALE for TALE repressor screening. A TALE targeting a 14bp sequence within the SOX2 locus of the human genome was synthesized as described previously . b, List of all repressors screened and their host origin (left). Eight different candidate repressor domains were fused to the C-term of the SOX2 TALE. c, The fold decrease of endogenous SOX2 mRNA is measured using qRT-PCR by dividing the SOX2 mRNA levels in mock transfected cells by SOX2 mRNA levels in cells transfected with each candidate TALE repressor. d, Transcriptional repression of endogenous CACNA1C. TALEs using NN, NK, and NH as the G-targeting RVD were constructed to target a 18bp target site within the human CACNA1C locus (site 1 in ). Each TALE is fused to the SID repression domain. NLS, nuclear localization signal; KRAB, Krüppel-associated box; SID, mSin interaction domain. All results are collected from three independent experiments in HEK 293FT cells. Error bars indicate s.e.m.; n = 3. * p < 0.05, Student’s t test.
Article Snippet: Truncation variants of the Krüppel-associated
Techniques: Sequencing, Synthesized, Quantitative RT-PCR, Transfection, Construct